Commission Regulation (EU) No 231/2012
law://eu/reg/2012/231/anx_1__grp_349@2026-11-12law://eu/reg/2012/231/anx_1__grp_349@2026-11-12| Synonyms | |||
| Definition | The manufacturing process comprises two main phases: the first involving water extraction of the leaves of the Stevia rebaudiana Bertoni plant and preliminary purification of the extract by employing ion exchange chromatography to yield a steviol glycoside primary extract, and the second involving recrystallisation of the steviol glycosides from methanol or aqueous ethanol resulting in a final product containing not less than 95 % of the below identified 11 related steviol glycosides, in any combination and ratio.The additive may contain residues of ion-exchange resins used in the manufacturing process. Several other related steviol glycosides that may be generated as a result of the production process, but do not occur naturally in the Stevia rebaudiana plant have been identified in small amounts (0,10 to 0,37 % w/w). | ||
| Chemical name | Steviolbioside: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acidRubusoside: 13-β-D-glucopyranosyloxykaur-16-en-18-oic acid, β-D-glucopyranosyl esterDulcoside A: 13-[(2-O-α–L-rhamnopyranosyl-β–D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl esterStevioside: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl esterRebaudioside A: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D- glucopyranosyl esterRebaudioside B: 13-[(2-O-β–D-glucopyranosyl-3-O-β–D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acidRebaudioside C: 13-[(2-O-α–L-rhamnopyranosyl-3-O-β–D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl esterRebaudioside D: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-β-D-glucopyranosyl esterRebaudioside E: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-β-D-glucopyranosyl esterRebaudioside F: 13[(2-O-β-D-xylofurananosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl esterRebaudioside M: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester | ||
| Molecular formula | Trivial name | Formula | Conversion factor |
| Steviol | C20 H30 O3 | 1,00 | |
| Steviolbioside | C32 H50 O13 | 0,50 | |
| Rubusoside | C32 H50 O13 | 0,50 | |
| Dulcoside A | C38 H60 O17 | 0,40 | |
| Stevioside | C38 H60 O18 | 0,40 | |
| Rebaudioside A | C44 H70 O23 | 0,33 | |
| Rebaudioside B | C38 H60 O18 | 0,40 | |
| Rebaudioside C | C44 H70 O22 | 0,34 | |
| Rebaudioside D | C50 H80 O28 | 0,29 | |
| Rebaudioside E | C44 H70 O23 | 0,33 | |
| Rebaudioside F | C43 H68 O22 | 0,34 | |
| Rebaudioside M | C56 H90 O33 | 0,25 | |
| Molecular weight and CAS No | Trivial name | CAS Number | Molecular weight (g/mol) |
| Steviol | 318,46 | ||
| Steviolbioside | 41093-60-1 | 642,73 | |
| Rubusoside | 64849-39-4 | 642,73 | |
| Dulcoside A | 64432-06-0 | 788,87 | |
| Stevioside | 57817-89-7 | 804,88 | |
| Rebaudioside A | 58543-16-1 | 967,01 | |
| Rebaudioside B | 58543-17-2 | 804,88 | |
| Rebaudioside C | 63550-99-2 | 951,02 | |
| Rebaudioside D | 63279-13-0 | 1129,15 | |
| Rebaudioside E | 63279-14-1 | 967,01 | |
| Rebaudioside F | 438045-89-7 | 936,99 | |
| Rebaudioside M | 1220616-44-3 | 1291,30 | |
| Assay | Not less than 95 % steviolbioside, rubusoside, dulcoside A, stevioside, rebaudiosides A, B, C, D, E, F and M on the dried basis, in any combination and ratio. | ||
| Description | White to light yellow powder, approximately between 200 and 350 times sweeter than sucrose (at 5 % sucrose equivalency). | ||
| Solubility | Freely soluble to slightly soluble in water | ||
| pH | Between 4,5 and 7,0 (1 in 100 solution) | ||
| Total ash | Not more than 1 % | ||
| Loss on drying | Not more than 6 % (105 °C, 2h) | ||
| Residual solvents | Not more than 200 mg/kg methanolNot more than 5000 mg/kg ethanol | ||
| Arsenic | Not more than 1 mg/kg | ||
| Lead | Not more than 1 mg/kg |
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