| Identification number of the additive | Name of the holder of authorisation | Additive | Composition, chemical formula, description, analytical method | Species or category of animal | Maximum age | Minimum content | Maximum content | Other provisions | End of period of authorisation |
|---|---|---|---|---|---|---|---|---|---|
| Units of activity/kg of complete feedingstuff with a moisture content of 12 % | |||||||||
| Category of zootechnical additives. Functional group: digestibility enhancers | |||||||||
| 4a40 | Genencor International B.V. | Endo- 1,4-beta-xylanase (EC 3.2.1.8), protease (EC 3.4.21.62) and alpha-amylase (EC 3.2.1.1) | Additive compositionPreparation of endo-1,4-beta-xylanase produced by Trichoderma reesei ATCCPTA-5588, protease produced by Bacillus subtilis CBS 148232 and alpha-amylase produced by Bacillus licheniformis ATCC SD-6525 with a minimum activity of:Endo-1,4-beta-xylanase: 20000 UXnote/gProtease: 40000 UPnote/gAlpha-amylase: 2000 UAnote/gSolid formCharacterisation of the active substanceEndo-1,4-beta-xylanase (EC 3.2.1.8) produced by Trichoderma reesei ATCCPTA-5588, protease (EC 3.4.21.62, also known as ‘subtilisin’) produced by Bacillus subtilis CBS 148232 and alpha-amylase (EC 3.2.1.1) produced by Bacillus licheniformis ATCC SD-6525Analytical methodnoteFor the quantification of endo-1,4-beta-xylanase in the feed additive, premixtures and compound feeds: colorimetric method, based on the enzymatic hydrolysis by xylanase of an azurine cross-linked wheat arabinoxylan substrate at pH 4,2 and 50 °C;For the quantification of protease in feed additive and premixtures and compound feeds: colorimetric method based on the enzymatic hydrolysis by protease of a dyed cross-linked casein substrate at pH 10,0 and 50 °C;For the quantification of alpha-amylase in feed additive, premixtures and compound feeds: colorimetric method based on the enzymatic hydrolysis by amylase of an azurine cross-linked starch polymer substrate at pH 8,0 and 40 °C. | Chickens for fatteningChickens reared for layingMinor poultry species for fattening and reared for laying | — | Endo-1,4-beta-xylanase 2000 UXProtease 4000 UPAlpha-amylase 200 UA | — | 1. In the directions for use of the additive and premixture, the storage conditions and stability to heat treatment shall be indicated.2. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal breathing, eye and skin protective equipment. | 28 September 2033 |
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