Commission Implementing Regulation (EU) 2023/1333

Identification number of the additive	Name of the holder of authorisation	Additive	Composition, chemical formula, description, analytical method	Species or category of animal	Maximum age	Minimum content	Maximum content	Other provisions	End of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a1603	Novozymes A/S	Endo-1,3(4)-beta-glucanase (EC 3.2.1.6)	*Additive compositionPreparation of endo-1,3(4)-beta-glucanase (EC 3.2.1.6) produced by Aspergillus fijiensis* CBS 589.94 with a minimum activity of:Coated form: 50 FBG^note/g.Liquid form: 120 FBG/mLCharacterisation of the active substanceEndo-1,3(4)-beta-glucanase produced by Aspergillus fijiensis CBS 589.94Analytical method^noteFor the quantification of 1,3(4)-beta-glucanase activity in the feed additive: colorimetric method measuring coloured compound produced by the dinitro salicylic acid (DNSA) based on the enzymatic hydrolysis of beta-glucan at pH 5,0 and 50 °C.For the quantification of 1,3(4)-beta-glucanase activity in premixtures and compound feed: colorimetric method measuring water soluble dyed fragments based on the enzymatic hydrolysis of cross-linked azo-barley-glucan at pH 4,5 and 50 °C.	Chickens for fatteningWeaned piglets	-	10 FBG	-	1. In the directions for use of the additive and premixture, the storage conditions and stability to heat treatment shall be indicated.2. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal breathing, eye and skin protective equipment.	20 July 2033