1.The genetically modified organisms to be placed on the market as product, hereinafter ‘the product’, are cut flowers of carnation (Dianthus caryophyllus L.), with modified flower colour, derived from a Dianthus caryophyllus L. cell culture, and transformed with Agrobacterium tumefaciens, strain AGL0, using the vector pCGP2355, and resulting in line 26407.The product contains the following DNA in three cassettes:
(a)Cassette 1The promoter from snapdragon chalcone synthase gene, the petunia cytochrome b5 (difF) cDNA encoding a cytochrome b5 protein to enhance activity of F3′5′H, and the terminator from a petunia gene encoding a phospholipid transfer protein homologue.
(b)Cassette 2The petunia flavonoid 3′5′-hydroxylase cDNA (f3′5′h) encoding F3′5′H, a key enzyme in the anthocyanin biosynthetic pathway and the promoter and terminator of a Dianthus caryophyllus anthocyanidin synthase (ans) gene.These two cassettes were inserted into the plant genome to obtain the desired flower colour.
(c)Cassette 3The Cauliflower mosaic virus 35S promoter, the 5'untranslated region of the petunia gene coding for chlorophyll a/b binding protein, the SuRB (als) gene coding for a mutant acetolactate synthase protein (ALS) derived from Nicotiana tabacum, which confers tolerance to sulfonylurea. This trait was used as a marker in the selection of transformants.
2.The consent shall cover progeny derived through vegetative reproduction of the genetically modified carnation (Dianthus caryophyllus L., line 26407).