27In that regard, it must be recalled that, according to well-established case-law of the Court, pursuant to Article 288 TFEU and by virtue of the very nature of regulations and of their function in the system of sources of EU law, the provisions of those regulations generally have immediate effect in the national legal systems without its being necessary for the national authorities to adopt measures of application. Nonetheless, some of those provisions may necessitate, for their implementation, the adoption of measures of application by the Member States (see, to that effect, judgment of 14 April 2011, Vlaamse Dierenartsenvereniging and Janssens, C‑42/10, C‑45/10 and C‑57/10, EU:C:2011:253, paragraphs 47 and 48 and the case-law cited).
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